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Exogenously-supplied PHS enhances acetic acid tolerance of S. cerevisiae. Serial dilution of otherwise wild-type cells expressing either +93xFLAG-Orm1 and 3xHA-Orm2 (JTY6140) or the 3xFLAG-Orm1(P*4) and 3xHA-Orm2(P*2) mutants were grown to mid-exponential phase and then serial dilutions were made and spotted onto MM4 agar plates containing the indicated acetic acid and PHS concentrations. Plates were imaged after 3 days of growth at 30 °C.
