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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Biochem J. 2016 Sep 26;473(23):4311–4325. doi: 10.1042/BCJ20160565

Table 1.

Yeast strains used in this study.

Strain Genotype Source/reference
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Research Genetics, Inc.
BY4742 MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Research Genetics, Inc.
YDB379 BY4741 Ypk1-3xFLAG::natNT2 J.S. Weissman, Univ. of
California, San
Francisco
yKL4 BY4741 TOR2+::Hygr [40]
yKL5z BY4741 Tor2(L2178A)::Hygr [40]
JTY6142 BY4741 ypk1Δ::KanMX4 Research Genetics, Inc.
YDB146 BY4741 3xFlag-Orm1 [41]
yAM159-A BY4741 3xFLAG-Lag1::LEU2 [40]
yAM163-A 3xFLAG-Lag1(S23A S24A)::LEU2 [40]
JTY6140 BY4741 MATa his3Δ1 leu2Δ0 met15Δ0
ura3Δ0 can1Δ::pSte2-His5 lyp1Δ::pSte3-
Leu5 3xFLAG-Orm1 3xHA-Orm2		 [41]
JTY5215 BY4741 MATa his3Δ1 leu2Δ0 met15Δ0
ura3Δ0 can1Δ::pSte2-His5 lyp1Δ::pSte3-
Leu5 3xFLAG-Orm1-P*4 3xHA-Orm2-P*2		 [41]
yAM205-A BY4742 Lac1::LEU2 Lag1::LEU2 [40]
yAM207-B BY4742 Lac1(S23A S24A)::LEU2
Lag1(S23A S24A)::LEU2 		[40]
yAM327 BY4741 Ypk1-3xFLAG hrk1Δ::kanMX4 This study
yAM336 BY4741 3xFlag-Orm1 hrk1Δ::kanMX4 This study
M24 BY4741 ipt1Δ::kanMX4 Research Genetics, Inc.
MTY175 BY4741, tetO7-AUR1::kanMX4 URA3 M. Tani, Kyushu
University, Japan