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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Lupus. 2016 May 26;26(1):62–72. doi: 10.1177/0961203316651738

Figure 6.

Figure 6

ART down-regulated MIF production through the inhibition of STAT1 phosphorylation. A, Protein levels of STAT1, 3, 5 and p-STAT1, 3, 5 in HUVECs with or without IFNα stimulation. B, Effect of ART treatment on IFNα induced pSTAT1 production. HUVECs were cultured with or without the presence of 1000U/ml IFNα for 24 hours and then those with IFNα stimulation were treated by 20 µM ART for 24 hours. C, Similar to that of fludarabine, a selective STAT1 inhibitor, ART treatment significantly down-regulated IFNα induced MIF production. D. Effect of ART treatment on IFNγ induced pSTAT1 production. HUVECs were cultured with or without the presence of 10ng/ml IFNγ for 24 hours and then those with IFNγ stimulation were treated by 20 µM ART for 24 hours. Each lane represented an independent sample and data were also shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.