FIGURE 4.

Electronic beam irradiation of S. ambofaciens strains deficient in NHEJ-like genes. Spores of mutants of NHEJ-like genes, namely single and multiple ku (A), ligase (B) and polymerase (C) mutants, were exposed to 0.1, 0.3, 0.5, 0.7, and 1 kGy of electronic beam irradiation. (D) In the same way, a Δku-like ΔrecA mutant also affected in HR was exposed to the same irradiation doses. In this latter case, a logarithmic scale was used for survival rate representation. Survival rates were calculated as the number of clones for a given dose relative to the number of clones unexposed to radiation. For each graph, wild-type strain is represented as a control. Each curve corresponds to data obtained from an individual mutant strain. Error bars indicate standard error of a minimum of three replicates.