Table 2.
Phenotypic characterisation of L. maculans resistance response for ILs, donor parents and other B. napus controls.
| L. maculans Isolatesa | B. napus Linesb | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dpi | Topas DH16516 | Topas-Rlm1 | Topas-Rlm2 | Topas-Rlm3 | Topas-Rlm4 | Topas-LepR1 | Topas-LepR2 | Topas-LepR3 | Westar | Quinta DH24288 | Columbus | Glacier DH24287 | Bristol | NLA51-1 | Quantum | AG-Castle | Scoop | Jet Neuf | Falcon | Roxet | Goéland | Darmor | 1065 | 1135 | Surpass 400 | NLA8-2 | |
| – | Rlm1 | Rlm2 | Rlm3 | Rlm4 | LepR1 | LepR2 | LepR3 | - | Rlm1 | Rlm1 | Rlm2 | Rlm2 | Rlm2 | Rlm3 | Rlm3 | Rlm4 | Rlm4 | Rlm4 | Rlm7 | Rlm9 | Rlm9 | LepR1 | LepR2 | LepR3 | LepR3 | ||
| Rlm3 | Rlm3 | Rlm3 | Rlm9 | RlmS | |||||||||||||||||||||||
| v23.1.3 | 10 | 7.9 | 2.7 | 7.9 | 7.1 | 2.9 | 2.5 | 6.5 | 1.4 | 8.0 | 3.6 | 2.5 | 7.2 | 7.7 | 7.2 | 6.7 | 7.9 | 1.2 | 2.6 | 2.9 | 3.5 | 7.0 | 7.5 | 2.8 | 7.9 | 2.5 | 1.8 |
| A1-A4-(5)-A6-A7-(8)-(10)-11*-J1*-(S)-AL1-AL3 | 14 | 9.0 | 3.0 | 8.9 | 9.0 | 2.9 | 3.5 | 8.2 | 2.1 | 9.0 | 5.2 | 3.6 | 8.9 | 8.9 | 9.0 | 8.8 | 8.8 | 1.8 | 2.9 | 3.2 | 4.4 | 9.0 | 9.0 | 5.9 | 5.6 | 3.6 | 2.2 |
| 98-15s | 10 | 7.8 | 8.0 | 3.0 | 8.0 | 2.9 | 2.8 | 3.1 | 7.6 | 7.8 | 8.0 | 7.4 | 3.1 | 3.9 | 2.3 | 6.5 | 7.0 | 2.6 | 2.8 | 2.7 | 3.3 | 7.9 | 7.6 | 3.3 | 6.6 | 4.3 | 8.2 |
| A2-A4-(5)-A6-A7-(8)-(10)-11*-J1*-(S)-AL1-AL2 | 14 | 9.0 | 9.0 | 3.2 | 9.0 | 3.0 | 3.7 | 3.8 | 9.0 | 9.0 | 9.0 | 9.0 | 3.7 | 4.8 | 3.0 | 8.7 | 8.3 | 2.7 | 2.9 | 2.8 | 3.3 | 8.7 | 8.5 | 7.0 | 8.1 | 6.9 | 9.0 |
| 00-100s | 10 | 7.9 | 8.3 | 2.3 | 2.5 | 7.9 | 2.4 | 3.0 | 8.0 | 7.9 | 3.0 | 2.5 | 2.2 | 2.8 | 1.7 | 2.4 | 1.8 | 7.8 | 6.3 | 6.3 | 6.7 | 3.3 | 3.3 | 2.9 | 4.2 | 2.8 | 8.0 |
| A2-A3-(5)-A6-(8)-A9-(10)-J1*-AS-AL1-AL2 | 14 | 8.8 | 9.0 | 2.8 | 3.2 | 8.9 | 2.9 | 3.1 | 8.9 | 9.0 | 4.0 | 3.4 | 2.5 | 3.0 | 2.6 | 3.0 | 2.7 | 9.0 | 8.0 | 7.4 | 7.8 | 3.9 | 4.1 | 3.2 | 5.5 | 3.1 | 9.0 |
| WA30s | 10 | 7.8 | 7.8 | 7.0 | 3.0 | 7.3 | 3.1 | 3.5 | 7.0 | 7.3 | 3.3 | 2.7 | 3.1 | 6.5 | 7.1 | 2.5 | 2.2 | 7.4 | 4.8 | 6.4 | 6.8 | 6.5 | 6.8 | 3.0 | 4.7 | 3.2 | 7.1 |
| A3-(5)-A6-(8)-(10)-J1*-AS-AL1-AL2 | 14 | 8.8 | 9.0 | 5.6 | 3.3 | 8.9 | 3.3 | 4.0 | 8.9 | 8.4 | 3.8 | 3.0 | 3.7 | 9.0 | 8.8 | 2.9 | 3.0 | 9.0 | 7.6 | 8.8 | 8.2 | 5.6 | 7.1 | 3.5 | 5.4 | 3.2 | 9.0 |
| 89-12s | 10 | 7.0 | 7.2 | 7.1 | 7.0 | 7.2 | 2.9 | 4.1 | 7.0 | 7.3 | 7.2 | 7.1 | 6.0 | 4.7 | 7.0 | 6.8 | 7.2 | 7.0 | 5.4 | 6.3 | 6.9 | 3.3 | 4.2 | 2.4 | 6.2 | 3.1 | 7.0 |
| (5)-A6-(8)-A9-(10)-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 9.0 | 9.0 | 8.9 | 3.2 | 4.5 | 9.0 | 8.9 | 8.8 | 9.0 | 8.3 | 5.7 | 8.9 | 8.8 | 5.6 | 9.0 | 7.9 | 8.3 | 7.9 | 3.9 | 5.0 | 4.1 | 7.3 | 3.1 | 9.0 |
| 05-31s | 10 | 7.6 | 8.1 | 3.0 | 7.8 | 7.1 | 3.3 | 4.0 | 7.0 | 8.0 | 8.0 | 8.0 | 3.1 | 3.3 | 2.9 | 7.6 | 7.3 | 7.1 | 4.1 | 4.7 | 3.5 | 6.8 | 7.6 | 6.3 | 6.3 | 3.4 | 7.6 |
| A2-(5)-A6-A7-(8)-(10)-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 3.4 | 9.0 | 8.3 | 4.1 | 4.6 | 9.0 | 9.0 | 8.9 | 9.0 | 4.1 | 3.8 | 3.1 | 8.9 | 9.0 | 9.0 | 7.1 | 7.3 | 4.0 | 8.2 | 8.8 | 8.4 | 7.7 | 3.9 | 9.0 |
| B14-13s | 10 | 7.7 | 8.1 | 3.0 | 7.8 | 7.6 | 8.0 | 3.9 | 8.2 | 8.1 | 8.2 | 8.1 | 3.0 | 3.0 | 2.9 | 7.2 | 7.3 | 8.0 | 4.8 | 4.9 | 5.9 | 3.3 | 4.3 | 8.0 | 6.7 | 3.1 | 8.0 |
| A2-(5)-A6-(8)-A9-(10)-11*-J1*-AS-AL2 | 14 | 9.0 | 9.0 | 3.2 | 9.0 | 9.0 | 9.0 | 4.4 | 9.0 | 9.0 | 9.0 | 8.9 | 3.7 | 3.0 | 3.0 | 8.9 | 8.4 | 9.0 | 6.4 | 6.4 | 7.5 | 3.9 | 5.1 | 9.0 | 7.7 | 3.1 | 9.0 |
| 3R11s | 10 | 6.7 | 7.0 | 6.8 | 6.9 | 6.8 | 3.1 | 4.2 | 6.9 | 7.3 | 7.0 | 6.8 | 6.5 | 7.0 | 6.9 | 7.3 | 7.1 | 7.0 | 5.6 | 5.5 | 4.0 | 6.9 | 6.9 | 4.1 | 5.9 | 4.2 | 7.0 |
| A7-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 9.0 | 9.0 | 8.9 | 3.4 | 4.8 | 9.0 | 8.8 | 8.8 | 9.0 | 8.9 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 8.0 | 8.3 | 4.5 | 8.5 | 8.3 | 7.0 | 9.0 | 6.6 | 9.0 |
| 3R11:AvrLm1 | 10 | 6.5 | 3.0 | 7.0 | 6.9 | 6.6 | 3.2 | 4.1 | 2.6 | 7.9 | 5.3 | 3.0 | 6.7 | 7.0 | 6.9 | 7.1 | 7.0 | 7.0 | 5.3 | 6.2 | 4.6 | 6.9 | 7.1 | 5.1 | 5.8 | 3.5 | 2.0 |
| A1-A7-11*-J1*-AS-AL1-AL2-AL3 | 14 | 8.5 | 3.2 | 9.0 | 8.8 | 8.3 | 3.4 | 4.8 | 3.0 | 9.0 | 7.0 | 4.6 | 5.6 | 8.9 | 9.0 | 9.0 | 9.0 | 9.0 | 7.9 | 9.0 | 5.3 | 9.0 | 8.8 | 7.5 | 9.0 | 4.2 | 2.7 |
| 3R11:AvrLm2 | 10 | 6.8 | 6.9 | 2.8 | 6.8 | 6.6 | 3.0 | 3.8 | 6.1 | 7.0 | 6.9 | 6.8 | 2.8 | 3.0 | 2.4 | 6.9 | 7.1 | 7.0 | 5.3 | 6.6 | 3.8 | 6.4 | 6.7 | 3.5 | 4.9 | 4.2 | 7.0 |
| A2-A7-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 2.9 | 9.0 | 8.3 | 3.6 | 4.4 | 9.0 | 8.8 | 8.5 | 9.0 | 3.0 | 3.1 | 2.9 | 9.0 | 9.0 | 9.0 | 7.8 | 7.6 | 4.1 | 8.1 | 8.1 | 6.4 | 8.8 | 5.8 | 9.0 |
| 3R11:AvrLm6 | 10 | 6.9 | 7.0 | 6.9 | 7.0 | 6.1 | 3.0 | 3.3 | 6.6 | 6.9 | 7.0 | 7.0 | 7.0 | 7.0 | 7.0 | 6.9 | 7.0 | 7.0 | 5.4 | 6.4 | 3.5 | 6.4 | 6.9 | 3.3 | 4.5 | 4.3 | 7.0 |
| A6-A7-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 9.0 | 9.0 | 5.6 | 3.4 | 3.9 | 9.0 | 8.5 | 8.8 | 9.0 | 8.5 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 7.1 | 8.0 | 3.8 | 8.0 | 8.8 | 5.8 | 8.8 | 6.9 | 9.0 |
| 2367s | 10 | 7.3 | 7.3 | 7.0 | 7.0 | 6.9 | 3.0 | 3.4 | 6.7 | 6.6 | 7.4 | 6.5 | 6.3 | 7.3 | 7.0 | 6.9 | 7.0 | 7.0 | 5.4 | 6.6 | 7.1 | 6.9 | 6.9 | 3.4 | 5.6 | 3.3 | 7.0 |
| A6-11*-J1*-AS-AL1-AL2 | 14 | 8.9 | 9.0 | 9.0 | 9.0 | 8.9 | 3.3 | 4.3 | 5.6 | 8.1 | 9.0 | 8.8 | 8.0 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 8.0 | 9.0 | 8.7 | 8.8 | 5.6 | 5.3 | 7.7 | 4.8 | 9.0 |
| 2367:AvrLm3 | 10 | 7.1 | 7.3 | 6.9 | 2.9 | 7.2 | 2.9 | 3.4 | 6.8 | 6.7 | 3.2 | 2.8 | 3.1 | 7.0 | 6.8 | 3.3 | 2.9 | 7.0 | 5.2 | 6.8 | 7.1 | 5.9 | 6.9 | 3.0 | 5.3 | 3.1 | 7.0 |
| A3-A6-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 8.9 | 3.9 | 9.0 | 3.4 | 4.4 | 8.7 | 8.5 | 4.1 | 3.0 | 4.3 | 9.0 | 9.0 | 3.3 | 3.1 | 9.0 | 7.5 | 9.0 | 8.8 | 8.5 | 8.7 | 3.7 | 8.4 | 4.6 | 9.0 |
| 2367:AvrLm4-7 | 10 | 7.3 | 7.0 | 7.0 | 7.0 | 3.0 | 3.1 | 3.8 | 7.3 | 7.1 | 7.5 | 6.4 | 6.7 | 7.2 | 7.0 | 7.1 | 7.0 | 3.0 | 2.8 | 3.0 | 3.1 | 7.0 | 6.9 | 3.0 | 6.8 | 4.8 | 7.1 |
| A4-A6-A7-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 8.9 | 9.0 | 3.1 | 3.6 | 4.6 | 9.0 | 5.6 | 9.0 | 8.5 | 8.8 | 8.9 | 9.0 | 9.0 | 9.0 | 3.3 | 2.9 | 3.0 | 3.7 | 8.8 | 8.8 | 4.4 | 8.7 | 5.9 | 9.0 |
| 2367:AvrLm7 | 10 | 6.9 | 7.1 | 7.0 | 7.0 | 6.4 | 3.0 | 3.5 | 7.1 | 6.8 | 7.5 | 6.8 | 6.6 | 7.0 | 6.9 | 6.9 | 7.0 | 7.0 | 5.8 | 6.6 | 3.0 | 7.0 | 7.1 | 3.1 | 6.3 | 5.0 | 7.0 |
| A6-A7-11*-J1*-AS-AL1-AL2 | 14 | 9.0 | 9.0 | 8.9 | 9.0 | 8.5 | 3.6 | 4.6 | 9.0 | 5.6 | 9.0 | 8.5 | 8.8 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 7.3 | 8.9 | 3.2 | 9.0 | 8.8 | 4.8 | 8.0 | 6.6 | 9.0 |
Shaded numbers represent averaged phenotypic rating (0–9 scale), dark green, most resistant; dark red, most susceptible,
a
native and transgenic L. maculans isolates followed by pathotype (A1–AS, AL1–AL3 = avirulent against Rlm1–RlmS, LepR1–LepR3, numbers in brackets denote interactions not determined, asterix denotes predicted phenotype based on genotyping, underlined entries denote avirulence conveyed by transgenic construct),
b
B. napus lines followed by known blackleg R gene content, bold entries denote introgressed R gene source.