Table 3.
Determination of Rlm3 and Rlm4 in B. napus varieties.
| L. maculans Isolateb | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 00-100s | v23.1.3 | 98-15s | WA30s | 89-12s | 05-31s | B14-13s | 2367s | 2367:AvrLm3 | 2367:AvrLm4-7 | 2367:AvrLm7 | ||||
| B. napus Linea | Reported R gene | Reference | Determined R genec | |||||||||||
| Topas DH16516 | none | 1 | 8.8 | 9.0 | 9.0 | 8.8 | 9.0 | 9.0 | 8.9 | 8.9 | 9.0 | 9.0 | 9.0 | none |
| Topas-Rlm3 | Rlm3 | this study | 3.2 | 9.0 | 9.0 | 3.3 | 9.0 | 9.0 | 9.0 | 9.0 | 3.9 | 9.0 | 9.0 | Rlm3 |
| Topas-Rlm4 | Rlm4 | this study | 8.9 | 2.9 | 3.0 | 8.9 | 8.9 | 8.3 | 9.0 | 8.9 | 9.0 | 3.1 | 8.5 | Rlm4 |
| Range | Rlm4 | 2 | 2.0 | 8.9 | 8.6 | 1.6 | 9.0 | 9.0 | 8.8 | 8.8 | 2.9 | 8.8 | 8.8 | Rlm3 |
| Maluka | LmR1 (Rlm4) | 2,3,4 | 2.1 | 8.9 | 8.1 | 3.2 | 9.0 | 9.0 | 8.9 | 8.5 | 3.0 | 9.0 | 8.8 | Rlm3 |
| Yickadee | none | 2 | 2.1 | 9.0 | 8.1 | 2.0 | 8.8 | 9.0 | 9.0 | 8.5 | 3.0 | 8.2 | 8.8 | Rlm3 |
| Q2 | Rlm3/Rlm3 + Rlm9 | 5,6,7,8 | 2.8 | 8.8 | 8.9 | 3.1 | 8.8 | 9.0 | 9.0 | 8.5 | 3.6 | 9.0 | 9.0 | Rlm3 |
| Shiralee | LmR1 (Rlm4) | 3,4,9 | 3.0 | 9.0 | 9.0 | 2.5 | 9.0 | 9.0 | 9.0 | 9.0 | 3.4 | 9.0 | 9.0 | Rlm3 |
| Dac-1 | unknown | 10,11 | 3.0 | 8.4 | 8.8 | 2.2 | 9.0 | 9.0 | 9.0 | 9.0 | 3.3 | 9.0 | 9.0 | Rlm3 |
| 46A65 | n/a | - | 3.2 | 8.8 | 9.0 | 3.0 | 9.0 | 9.0 | 9.0 | 9.0 | 4.1 | 9.0 | 9.0 | Rlm3 |
| SP-Banner | n/a | - | 3.4 | 9.0 | 9.0 | 2.9 | 8.9 | 9.0 | 8.9 | 9.0 | 4.2 | 9.0 | 9.0 | Rlm3 |
| Cresor | LmFr1 (Rlm4) | 4,9,12 | 3.5 | 9.0 | 8.9 | 2.9 | 9.0 | 9.0 | 9.0 | 8.8 | 3.6 | 8.8 | 9.0 | Rlm3 |
| Major | LEM1 (Rlm4) | 4,13,14 | 3.7 | 9.0 | 9.0 | 4.0 | 9.0 | 9.0 | 9.0 | 9.0 | 4.4 | 9.0 | 9.0 | Rlm3 |
| Defender | n/a | - | 4.1 | 9.0 | 9.0 | 3.5 | 8.9 | 9.0 | 9.0 | 9.0 | 4.5 | 9.0 | 9.0 | Rlm3 |
| Sprint | n/a | - | 4.2 | 9.0 | 9.0 | 3.5 | 8.4 | 9.0 | 9.0 | 9.0 | 4.4 | 9.0 | 9.0 | Rlm3 |
| Garrison | n/a | - | 4.2 | 8.8 | 8.6 | 3.5 | 8.6 | 9.0 | 9.0 | 9.0 | 4.3 | 9.0 | 9.0 | Rlm3 |
| AG-Spectrum | polygenic | 15 | 4.3 | 9.0 | 9.0 | 3.4 | 9.0 | 9.0 | 9.0 | 9.0 | 3.9 | 9.0 | 9.0 | Rlm3 |
| DH12075 | CLmR1 (Rlm4) | 9,16 | 4.4 | 9.0 | 9.0 | 3.8 | 9.0 | 9.0 | 9.0 | 9.0 | 4.8 | 9.0 | 9.0 | Rlm3 |
| Sentry | unknown | 8 | 4.6 | 8.8 | 9.0 | 4.5 | 8.5 | 9.0 | 9.0 | 9.0 | 4.9 | 9.0 | 9.0 | Rlm3 |
| AC-Excel | n/a | - | 6.0 | 9.0 | 9.0 | 5.5 | 9.0 | 9.0 | 9.0 | 9.0 | 4.6 | 9.0 | 9.0 | Rlm3 |
| Capitol | Rlm1, Rlm3 | 2,17 | 3.6 | 4.9 | 8.1 | 2.6 | 8.4 | 9.0 | 8.5 | 8.8 | 3.8 | 9.0 | 9.0 | (Rlm1/LepR3), Rlm3 |
| Dunkeld | Rlm4/poly/Rpg3Dun | 2,5,15,18,19 | 8.9 | 3.0 | 3.0 | 7.8 | 7.8 | 8.5 | 9.0 | 8.5 | 8.0 | 2.9 | 8.0 | Rlm4 |
| Monty | Rlm4 | 2 | 8.6 | 3.0 | 2.4 | 8.5 | 8.8 | 8.8 | 8.1 | 9.0 | 9.0 | 3.0 | 7.8 | Rlm4 |
| Pollen | Rlm4 | 2,20 | 8.8 | 3.0 | 2.9 | 8.0 | 9.0 | 7.8 | 8.4 | 9.0 | 9.0 | 3.0 | 8.8 | Rlm4 |
| Cooper | Rlm1, Rlm4 | 21 | 8.6 | 3.1 | 3.1 | 8.2 | 9.0 | 8.8 | 8.8 | 9.0 | 9.0 | 3.0 | 8.8 | (Rlm1/LepR3), Rlm4 |
| AV-Sapphire | Rlm4/Rlm4 + Rlm9 | 5,15,22 | 9.0 | 3.4 | 3.0 | 6.8 | 6.5 | 8.8 | 6.9 | 8.5 | 8.0 | 3.1 | 7.5 | Rlm4 |
| Trigold | n/a | - | 8.5 | 3.5 | 3.2 | 8.5 | 9.0 | 9.0 | 8.5 | 8.8 | 9.0 | 2.6 | 8.8 | Rlm4 |
| Val-1 | unknown | 10,11 | 9.0 | 3.8 | 3.1 | 8.8 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 3.5 | 9.0 | Rlm4 |
| PSA12 | n/a | - | 9.0 | 9.0 | 9.0 | 8.6 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | - |
| Cadillac | Rlm1 | 23 | 8.0 | 4.0 | 9.0 | 8.5 | 8.0 | 9.0 | 8.8 | 9.0 | 9.0 | 9.0 | 9.0 | (Rlm1/LepR3) |
| AV-Garnet | Rlm1, Rlm9 | 5 | 4.8 | 4.9 | 9.0 | 8.4 | 5.8 | 9.0 | 7.7 | 9.0 | 9.0 | 9.0 | 9.0 | (Rlm1/LepR3, Rlm9) |
| Samourai | Rlm2, Rlm9 | 20 | 2.6 | 8.2 | 3.2 | 7.4 | 4.2 | 3.2 | 2.9 | 8.2 | 7.8 | 8.0 | 8.4 | Rlm2*, (Rlm9) |
| Tapidor DH | Rlm2/Rlm2 + Rlm4 | 2,24 | 2.0 | 8.0 | 2.8 | 7.9 | 3.3 | 3.6 | 2.9 | 8.8 | 8.6 | 8.8 | 8.2 | Rlm2*, (Rlm9) |
| Avisio | Rlm9 | 25 | 4.0 | 9.0 | 9.0 | 9.0 | 4.9 | 8.8 | 5.0 | 8.8 | 8.5 | 9.0 | 8.8 | (Rlm9) |
Shaded numbers represent averaged phenotypic rating (0–9 scale), dark green, most resistant; dark red, most susceptible.
B. napus variety lines and previously-reported R gene content. References: 1, (Larkan et al., 2013); 2, (Rouxel et al., 2003b); 3, (Mayerhofer et al., 1997); 4, (Delourme et al., 2006); 5, (Marcroft et al., 2012); 6, (Van De Wouw et al., 2009); 7, (van de Wouw et al., 2014); 8, (Zhang et al., 2015); 9, (Mayerhofer et al., 2005); 10, (Kutcher et al., 2007); 11, (Moreno-Rico et al., 2001); 12, (Dion et al., 1995); 13, (Ferreira et al., 1995); 14, (Rouxel et al., 2003b); 15, (Raman et al., 2012b); 16, (Yu et al., 2012); 17, (Balesdent et al., 2002); 18, (Li et al., 2004); 19, (Dusabenyagasani and Fernando, 2008); 20, (Balesdent et al., 2005); 21, (Kutcher et al., 2010); 22, (Larkan et al., 2016); 23, (Gout et al., 2006); 24, (Ghanbarnia et al., 2015); 25, (Light et al., 2011).
Native and transgenic L. maculans isolates, pathotypes as described in Table 2.
R gene content of each line as determined in this study; genes in brackets not confirmed in this test, asterix denotes genes previously confirmed through cloning (Larkan et al., 2015).