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. 2016 Nov 28;9:131. doi: 10.3389/fnmol.2016.00131

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Copyright © 2016 Yang, Wu, Zhang and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calpeptin pretreatment blocked the protective effect of PC in vivo. (A) Representative images of HDAC3 subcellular localization in cortex. Scale bar: 50 μm. (B) Representative images of TTC staining from each group are shown. Infarct volume percentage from each slice were determined (n = 8–10). ^∗p < 0.05 versus MCAO group, ^#p < 0.05 versus PC group, ANOVA. (C) Representative images of Fluoro-Jade C staining and the numbers of degenerating neurons (over 30 slices from six rats per group). ^∗∗∗p < 0.001 versus MCAO group, ^###p < 0.001 versus PC group, ANOVA. (D) Expression levels of Prdx-2, Bcl-xL and HSP70 in each group (n = 3–4) 24 h after MCAO. ^#p < 0.05, ^###p < 0.001 versus Sham group; ^∗p < 0.05, ^∗∗p < 0.01 versus MCAO group, ANOVA. (E) Representative images of Cerebral HSP70 immunohistochemistry. Scale bar: 50 μm. Calp, calpeptin.