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Selected pixel numbers of immunohistochemical staining with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling (TUNEL) and glial fibrillary acidic protein (GFAP) at each time point are showing that TUNEL-positive cells in the injured hemisphere are observed from 1 day, peak at 3 days and then decline by 21 days. Selected pixel numbers for GFAP-positive cells are showing an increase from 1 day to 21 days.
