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. 2016 Nov 28;2:16080. doi: 10.1038/cddiscovery.2016.80

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Involvement of MICU1 in intestinal barrier integrity in vitro. (a) Clearance of FSA. (b) Immunofluorescence analysis on tight junction proteins, claudin 1, occludin, and ZO-1 (green). (c) Western blotting analysis of tight junction proteins. Results are shown as mean±S.D. of three independent experiments. Statistical analysis was performed by one-way ANOVA test and independent t-test. versus control, *P<0.05; versusNC, ^#P<0.05. NC (negative control group), Caco-2 cells transfected with empty lentiviral vector. MICU1-KD, MICU1-knockdown.