Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 28;6:37828. doi: 10.1038/srep37828

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Schema indicates the regions of intercusps (I) and cusps (II), from which the images were taken for (b–g and h–m) respectively. (b–m) Effects of NSC23766 on F-actin, E-cad and FN in the IDE of gerbils in intercuspal regions (b–g) and in cuspal regions (h–m). Red indicates the nuclei. (b,e,h,k) Equal levels of F-actin assembled in the apex and base of IDE cells (b,h; arrows) were disrupted (e,k; arrows) after NSC23766 treatment. Z in panel b and e indicates the vertical view. (c,f,i,l) E-cad was slightly increased both in the cuspal and intercuspal regions after NSC23766 treatment. The graphic images of cytoplasm (green) and nucleus (red) inserted in panel c and f are 3D reconstructions of the outlined cells, showing the cell shapes and nuclear localization. The cells had a triangular shape in the intercuspal region and a round-compact shape in cuspal regions. (d,g,j,m) The unique assembly of FN in the basement membrane along the IDE sheet was disrupted by NSC23766. The localization of FN increased both in the intercuspal (g; arrow) and cuspal regions (m; arrow) compared with the control (d,j; arrows), but lost its assembly in the basement membrane in the cuspal regions (m; arrowhead). The formation of a pit in the IDE after several IDE cells overexpressed EGFP-RhoA-Q63L by electroporation. Green fluorescence indicates individual cells expressing RhoA-Q63L (n). RhoA-Q63L-expressing cells (n) had enhanced expression of F-actin (o) and E-cad (p) compared with neighboring cells. (q–s) FN (red) deposited around the side of RhoA-Q63L-expressing IDE cells (arrows). Blue indicates nuclei. White dashed lines outline the IDE. IDE, inner dental epithelium. Scale bar, 20 μm.