Table 3. Comparison of variants allele frequency (VAF) for mutations in CTCs detected by targeted sequencing (VAF 1) and digital PCR (VAF 2).

Patient #	Gene	Genomic Position	Ref/Alt	VAF 1 (%)	VAF2 (%)
20	EGFR	Chr7_55259599	G873R	3.9	0.0
	ATM	Chr11_108236062	Q3000*	4.3	N.A.a
	TP53	Chr17_7578260	V179M	7.6	7.5
	SMAD4	Chr18_48584594	Q256L	4.5	0.0
22	EGFR	Chr7_55249081	M793I	20.0	17.8
24	MET	Chr7_116411923	R988C	7.5	7.2
	FGFR2	Chr10_123274803	S372F	3.3	2.6
27	FGFR2	Chr10_123279539	G298D	5.1	4.5
32	FGFR3	Chr4_1806270	R399C	6.1	1.7b
	TP53	Chr17_7579442	P82L	4.7	4.1
36	ALK	Chr2_29443646	P1191S	12.2	12.1

^aData not available because a TaqMan probe for this mutation was not prepared.

^bMutation also detected in matched WBCs.