. 2016 Nov 28;6:37836. doi: 10.1038/srep37836

Table 4. PCR primers used in this study.

Name	Site	Primer sequence (5′–3′)	Annealing temp. (°C)	Product size (bp)	CpGs (n)
CpG island-1	−1337~−1059	F: TTTAATATTTTGGTTAATATGGGTGAAATTT	70.4	279	14
CpG island-1	−1337~−1059	R: AAAACTCCCAAAACACTCATCTATC	70.4	279	14
CpG island-2	−163~ +76	F: TTTATAATTTAGAGGAGGTTGGGTT	70.1	250	12
CpG island-2	−163~ +76	R: CTACCCTCTTTTCCCACCTTAATATA	70.1	250	12
CpG island-3	+123~ +476	F: TTTTTGGGTTGGGAAAGAGA	70.4	354	37
CpG island-3	+123~ +476	R: AACTACTAAACCTTAAATCAAACAAC	70.4	354	37
CpG island-4	+630~ +953	F: TTTAAGGTTTTGTTTGGGAGAGGATAG	69.8	324	35
CpG island-4	+630~ +953	R: ACTAAAATCAAAAAACCAAAAAAAA	69.8	324	35
CpG island-5	+933~ +1229	F: TTTTGTTTTTTTGATTTTAGTTTAT	70.0	297	20
CpG island-5	+933~ +1229	R: AACCTTAATTCCCCATTTAAAATCC	70.0	297	20
VDAC2	—	F: AGAGAAGCGGTCCCTCAGAA	68.9	100	—
VDAC2	—	R: AAACAAAGCCCTGCATAGGGT	68.9	100	—
β-actin	—	F: TCACCCACACTGTGCCCATCTACGA	72.3	295	—
β-actin	—	R: CAGCGGAACCGCTCATTGCCAATGG	72.3	295	—