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. 2016 Nov 28;6:37943. doi: 10.1038/srep37943

Figure 3. Effect of AhNAC2 or AhAREB1 on pAhNCED1::LUC activity during ABA treatment.

Figure 3

(a) Schematic of NACRE and ABRE mutations. The triangle represents mutation of the core element. The core sequences of the NACRE and ABRE elements were mutated to AAAA. (b) LUC activity was measured by transient expression of pAhNCED1::LUC and p35S::AhNAC2 or p35S::AhAREB1 vectors in Arabidopsis protoplasts. A 5 μg quantity of purified vector was used per transfection. Protoplasts were incubated overnight in W5 buffer. After transfection, protoplasts were incubated overnight. (c) Coordinate regulation of pAhNCED1::LUC activity by co-expression of AhNAC2 and AhAREB1 with and without ABA treatment. Error bars indicate SEM (n = 3).