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. 2016 Nov 28;6:37943. doi: 10.1038/srep37943

Figure 4. EMSA of AhNAC2 and AhAREB1 protein binding to the core NACRE element in the AhNCED1 promoter.

Figure 4

(a) Schematic of the position of the probes in the AhNCED1 promoter. A 30 bp DNA fragment containing CACGTG was designed. WT: probe containing CACGTG; MU: probe changing CACG to AAAA. (b) Both AhAREB1 and AhNAC2 can bind to the same NACRE element. (c) Sequential addition of the two TFs into the reaction system. The black arrow indicates binding of AhNAC2 and the green arrow indicates binding of AhAREB1 to the promoter of AhNCED1; ‘Comp DNA’ indicates addition of competitor DNA.