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. 2016 Nov 28;22(44):9765–9774. doi: 10.3748/wjg.v22.i44.9765

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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Oxidative stress assay in vitro. A: Electron spin resonance (ESR) spectroscopy performed 30 min after linalool treatment. The signal intensity of 1:2:2:1 suggests generation of hydroxyl radical, the strongest reactive oxygen species (ROS); B: The signal of HCT 116 without linalool at the same time period; C: Diphenyl-1-pyrenylphosphine (DPPP) assay performed to detect lipid peroxidation showed that linalool induced cell membrane lipid peroxidation as green fluorescent on the cell surface(white arrow); D: The cell membrane lipid peroxidation was not observed in without linalool treatment group.