Table 1. Genomic features of the ICEs, antibiotic resistance patterns and transfer frequency of ICE-contained strains in this study.
| Strain ID | Location | Species | Sample source | Year of isolation | ICE name | ICE length (bp) | G + C (%) | No. of predicted CDSs (RAST) | Antibiotic resistance phenotype | Transfer frequency | SXT/R391 ICE gene |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dfhR | floR | strB/A | sul2 | tetA/R | |||||||||||
| 08MAS2213 | Maanshan | P. vulgaris | food | 2008 | ICEPvuCHN2213 | 94,340 | 46.8 | 92 | AMP-AZM-CHL-CFZ-SAM-STR-SXT-SUL | 1.0 × 10−5 | − | + | + | + | − |
| 08MAS1586 | Maanshan | P. mirabilis | food | 2008 | ICEPmiCHN1586 | 99,355 | 46.6 | 85 | AMP-AZM-CHL-CIP-SAM-STR-SXT-SUL-TCY | 2.5 × 10−2 | + | + | + | + | − |
| 09MAS2407 | Maanshan | P. mirabilis | stool | 2009 | ICEPmiCHN2407 | 97,078 | 47 | 95 | AMP-AZM-CHL-CFZ-CIP-KAN-STR-SXT-SUL-TCY | 9.5 × 10−4 | − | − | − | − | + |
| 09MAS2410 | Maanshan | P. mirabilis | stool | 2009 | ICEPmiCHN2410 | 93,537 | 46.5 | 86 | AMP-AZM-CHL-CFZ-CIP-KAN-STR-SXT-SUL-TCY | NT | − | − | − | − | − |
| 09MAS2416 | Maanshan | P. mirabilis | stool | 2009 | ICEPmiCHN2416 | 92,556 | 46.7 | 86 | AMP-AZM-CHL-CFZ-CIP-GEN-KAN-STR-SXT-SUL-TCY | NT | − | − | − | − | − |
| MD20140901 | Beijing | P. mirabilis | stool | 2014 | ICEPmiCHN901 | 89,493 | 47.3 | 88 | AMP-AZM-ATM-AMK-CHL-CXM-CFZ-CRO-CIP-GEN-SAM -KAN-STR-SXT-SUL-TCY | NT | + | + | + | + | − |
| MD20140902 | Beijing | P. mirabilis | stool | 2014 | ICEPmiCHN902 | 89,096 | 47.1 | 86 | AMP-AZM-ATM-AMK-CHL-CXM-CFZ-CRO-CIP-SAM-KAN-STR-SXT-SUL-TCY | NT | + | + | + | + | − |
| MD20140903 | Beijing | P. mirabilis | stool | 2014 | ICEPmiCHN903 | 89,644 | 47.3 | 86 | AMP-AZM-AMK-CHL-CXM-CFZ-CRO-CIP-SAM -KAN-STR-SXT-SUL-TCY | NT | + | + | + | + | − |
| MD20140904 | Beijing | P. mirabilis | stool | 2014 | ICEPmiCHN904 | 94,942 | 47.7 | 99 | AMP-AZM-ATM-AMK-CHL-CXM-CFZ-CRO-CIP-FEP-SAM -KAN-STR-SXT-SUL-TCY | NT | − | + | + | + | + |
| MD20140905 | Beijing | P. mirabilis | stool | 2014 | ICEPmiCHN905 | 94,956 | 47.8 | 98 | AMP-AZM-ATM-AMK-CHL-CXM-CFZ-CRO-CIP-GEN-SAM -KAN-STR-SXT-SUL-TCY | NT | − | + | + | + | + |
| TJ1809 | Tianjin | P. mirabilis | stool | 2013 | ICEPmiCHN1809 | 76,218 | 47.1 | 67 | AMP-AZM-CHL-CIP-KAN-SXT-SUL-TCY | 4.0 × 10−5 | − | − | − | − | − |
| TJ3237 | Tianjin | P. mirabilis | stool | 2013 | ICEPmiCHN3237 | 87,215 | 46.8 | 74 | AMP-AZM-CFZ-SXT-SUL-TCY | NT | − | − | − | − | − |
| TJ3277 | Tianjin | P. mirabilis | stool | 2013 | ICEPmiCHN3277 | 104,175 | 46.7 | 53 | AMP-AZM-CFZ-SXT-SUL-TCY | NT | NT | NT | NT | NT | NT |
| TJ3300 | Tianjin | P. mirabilis | stool | 2013 | ICEPmiCHN3300 | 108,335 | 46.7 | 98 | AZM-CHL-STR-SXT-SUL | NT | + | + | + | + | − |
| TJ3335 | Tianjin | P. mirabilis | stool | 2013 | ICEPmiCHN3335 | 89,996 | 47.8 | 84 | AZM-CHL-KAN-STR-SXT-SUL-TCY | 5.0 × 10−6 | − | + | + | + | − |
Abbreviations: AMP: ampicillin, AZM: azithromycin, CFZ: cefazolin, CXM: cefuroxime, CRO: ceftriaxone, FEP: cefepime, CTT: cefotetan, ATM: aztreonam, IPM: imipenem, SAM: ampicillin-sulbactam, TZP: piperacillin-tazobactam, AMK: amikacin, GEN: gentamycin, KAN: kanamycin, STR: streptomycin, SUL: sulfamethoxazole, SXT: trimethoprim-sulfamethoxazole, CIP: ciprofloxacin, CHL: chloramphenicol, TCY: tetracycline. Abbreviations in bold indicated positive resistance phenotype but negative for their gene at ICE. “+”: positive, “−”: negative, “NT”: not test.