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Western blot from 12% SDS–PAGE of TCA-precipitated supernatant (TCA-S1), cell pellet (P1) and cell lysate (S2) obtained following treatment of High Five TM cells with either 1 construct alone 2 construct and Cellfectin® and 3 Cellfectin® alone for 3 days. X rApp1 control. Secreted protein is observed in TCA-precipitated supernatant from cells treated with construct and Cellfectin®, and cell lysate of the same treatment
