Figure 3. Hyperplasia of the pancreatic mesenchyme upon elevated Hh signaling.

(A) Histological analysis of pancreatic tissue of e18.5 Nkx3.2-Cre;Ptch^flox/flox transgenic (right) and non-transgenic (Cre negative; ‘Non tg’; left) littermate embryos. Tissue-sections stained with Hematoxylin and Eosin (H&E). (B) Pancreatic tissues of e18.5 Nkx3.2-Cre;Ptch^flox/flox transgenic and non-transgenic (Cre negative; ‘Non tg’) littermate embryos were stained with antibodies against Desmin (‘Des’; green) and Amylase (‘Amy’; red) and counterstained with DAPI (blue). Shown are representative fields. (C) Immunofluorescence analysis of dissected pancreatic tissues from Nkx3.2-Cre;R26-YFP;Ptch^flox/flox (right) and Nkx3.2-Cre;R26-YFP;Ptch^flox/+ (left) control littermate e18.5 embryos. Tissues were stained with antibodies against YFP (green), α smooth muscle actin (αSMA; red) and counterstained with DAPI (blue). C’) Higher magnification of areas framed in a white box in (C) showing the αSMA and DAPI channels. Shown are representative fields.