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Twist1 mRNA expression in mouse blastocysts. Significant differences were observed in the presence of high glucose concentration (52 mM d-Glu), where the Twist1 mRNA levels were remarkably increased in blastocysts compared with the other groups (*P < 0.001). N = 30 embryos per group. MDA-MB-231 (breast cancer cell line) was used as a positive control for Twist1. Cntrl control blastocyst, NC negative control
