Expression of Asef, Tom1l1, Mfge8, and Gpr37l1 in astrocyte precursors. A–D, ISH demonstrating expression of Asef in the developing spinal cord. E, F, Coexpression of Asef with Glast and GFAP. G–J, ISH demonstrating expression of Tom1l1 in the developing spinal cord. K, L, Coexpression of Tom1l1 with Glast and GFAP. M–P, ISH demonstrating expression of Mfge8 in the developing spinal cord. Q, R, Coexpression of Mfge8 with Glast and GS. S–V, ISH demonstrating Gpr37l1 expression in the developing spinal cord. W, X, Coexpression of Gpr37l1 with Glast and GFAP. Dashed boxed in B, H, and N correspond to E, K, and Q, respectively. Dashed boxes in D, J, P, and V correspond to F, L, R, and X respectively. Note that W corresponds to P4 spinal cord. Arrows denote coexpression of a given gene with the listed marker.