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. 2016 Nov 23;36(47):11904–11917. doi: 10.1523/JNEUROSCI.1658-16.2016

Figure 5.

Figure 5.

Asef regulates astrocyte differentiation in developing spinal cord. A–L, Immunofluorescence staining of astrocyte lineage markers at E18.5 in Aldh1l1-GFP;Asef+/− and Aldh1l1-GFP;Asef−/− mice. Aldh1l1-GFP;Asef−/− mice demonstrate reduced AQP4 (C, D vs I, J) and SNTA1 expression (E, F vs K, L) compared with Aldh1l1-GFP;Asef+/− mice. M–O, R–T, ISH of astrocyte precursor (Glast; M–R) and oligodendrocyte lineage makers (N–S, O–T) at E18.5 in Aldh1l1-GFP;Asef+/− and Aldh1l1-GFP;Asef−/− mice. P, U, Immunofluorescence staining of pericytes markers at E18.5. PDGFRβ expression is no different in Aldh1l1-GFP;Asef+/− and Aldh1l1-GFP;Asef−/− mice. Q, V, Immunofluorescence staining of β-DAG at E18.5 show no difference between Aldh1l1-GFP;Asef+/− and Aldh1l1-GFP;Asef−/− mice. Dashed boxes are blown up in adjacent panels and demonstrate colocalization of β-DAG in Aldh1l1-GFP-expressing cells.