Figure 6.

BBB is compromised in the adult CNS in the absence of Asef. A, Analysis of the relative amount of Evans blue extracted from the spinal cord or cortex from P20 or 6-month-old mice. In each case, for each genetic condition and time point, Evans blue was extracted and quantified as μg/μl/mg of tissue. The values presented are a relative comparison between Asef^+/− and Asef^−/−. N > 4 for each genetic condition, at each time point. B, Immunoblot demonstrating coimmunoprecipitation of SNTA and ASEF from 293 cells transfected with tagged versions of these proteins, with and without RAC transfection. Error bars in A are SEM and *p = 0.04 and **p = 0.007.