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. 2016 Nov 28;6:37720. doi: 10.1038/srep37720

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The comparison of ionization efficiency of selected peptide obtained from peptic digest of ubiquitin: (a) containing TPP⁺ tag b. without ionization tag (peptide labeled with ¹³C isotope) The experiment was performed in LC-MS mode. The given mass spectra corresponds to two chromatographic peaks. The spectrum presented in panel a corresponds to the AGKQLEDGRTLSD peptide tagged at Lys moiety, while the spectrum in panel (b) – to the unmodified peptide. The details of experiment were given in Results and Discussion.