Figure 7. Greater chlamydial burden in LNG-treated mice elicits increased mononuclear cell inflammation.

In separate studies, groups of mice were treated and infected identically as described in Fig. 6. Mice were euthanized at 12 dpi, and pulmonary tissue processed to define inflammation by histology. (a) Representative results from hematoxylin and eosin, IHC, and IF staining depicts the increased mononuclear cell inflammation seen in the lungs of LNG-treated- and CD4⁺ cell-depleted mice; IF staining: CD11b⁺ (green), DAPI (blue); black and white bars denote 200 μm and 20 μm, respectively. (b) Quantification of pulmonary CD45⁺ inflammatory aggregates identified by IHC staining (5 high-power fields examined per section); (bars denote mean ± S.D.). Data are from 2 independent experiments with 6 mice per group. Statistical analyses were performed using 1-way ANOVA with Dunnett’s multiple comparisons test.