Skip to main content
. 2016 Nov 28;6:37758. doi: 10.1038/srep37758

Figure 3. Eno/eRNA interaction depends on the size of the nucleic acids and on their nucleotide composition.

Figure 3

(A,B) Total RNA and DNA were isolated from human A549 cells and subjected to different sonication times (1–12 s). Resulted eRNA (A) and DNA (B) was resolved on the agarose gel. (C) The binding of eRNA to pneumococcal Eno was determined using the solid-phase binding assay. Five μg of immobilized Eno were incubated with 10 μg sonicated eRNA. Eno/eRNA complexes were detected by chemiluminescence. Data represent mean values ± SEM; n = 3; **p ≤ 0.01; *p ≤ 0.05 vs control; ##p ≤ 0.001 vs 10 s of sonication. (D) The binding of eDNA to pneumococcal Eno was determined using the solid-phase binding assay. Five μg of immobilized Eno were incubated with 10 μg sonicated and biotinylated eDNA. Eno/eDNA complexes were detected by chemiluminescence. Data represent mean values ± SEM; n = 3; ***p ≤ 0.001 vs control. (E) Five  μg of synthetic oligonucleotides (polydIdC, AU, polyC, polyIC, GC), eRNA and eDNA were immobilized on the nitrocellulose membrane and their binding ability to biotinylated pneumococcal Eno was tested by dot blot. (F) Densitometry of (E). Data represent mean values ± SEM; n = 3.