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. 2016 Nov 28;6:37758. doi: 10.1038/srep37758

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(A) Two hundred fifty μg of cell wall proteins isolated from S. pneumoniae serotype 2 strain (ATCC11733), were resolved using two-dimensional gel electrophoresis. Polyacrylamide gels were simultaneously subjected to Western blotting using biotinylated eRNA (left panel) and to flamingo fluorescent gel stain (right panel). (B) List of proteins identified by MALDI-TOF analysis.