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. Author manuscript; available in PMC: 2017 Sep 14.
Published in final edited form as: Sci Transl Med. 2016 Sep 14;8(356):356ra119. doi: 10.1126/scitranslmed.aad9943

Figure 6. IL-6 upregulates inflammatory homing makers CXCR6 and CCR5 on TEM cells.

Figure 6

Purified CD3 T cells were cultured in the absence or presence of IL-6 (10 ng/ml) for 48 hours, followed by surface staining and flow cytometric analysis. (A and B) Effect of IL-6 on frequency of CXCR6+ (A) and CCR5+ (B) CD4 and CD8 TEM cells. (C and D) Effect of IL-6 on CD62L (C) and CCR7 (D) expression in naïve (CD45RA+) and memory (CD45RA−) CD4 T cells. (E) Linear regression analysis demonstrating the relationship between IL-6/pSTAT3 and baseline CD62L (left panel) and IL-6-induced CD62L (right panel) in CD4 naïve T cells; n=7 (Ctrl) and n=11 (T1D) in all panels. Statistical tests: Wilcoxon matched pairs, Mann Whitney U; r = Pearson correlation coefficient.