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. Author manuscript; available in PMC: 2016 Nov 28.
Published in final edited form as: ACS Chem Neurosci. 2016 Aug 24;7(9):1188–1191. doi: 10.1021/acschemneuro.6b00215

Figure 1.

Figure 1

Stabilized nuclear HIF1α is robustly expressed after hypoxia. (A) Primary neuronal cultures (DIV21–25) were exposed to normal growth media (control) or to 15 min OGD. Neurons were harvested 6 h after OGD and prepared for subcellular fractionation. Nuclear and cytosolic fractions were prepared for Western blotting for HIF1α, a hypoxia-induced protein, and β-tubulin, a cytosolic loading control. Western blot analysis demonstrates that 15 min of OGD induces HIF1α protein expression and nuclear stabilization 6 h post-OGD. Normoxic control cultures exhibit little to no cytosolic or nuclear HIF1α expression. Quantification of band intensity reveals a 5-fold increase in nuclear HIF1α after OGD compared to control (C, n = 4, p = 0.007, *denotes significance). No significant difference in β-tubulin expression was observed (B, n = 4, p = 0.4).