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. 2016 Feb 5;20(4):721–730. doi: 10.1111/jcmm.12785

Figure 1.

Figure 1

Heterogeneous expression of cell surface proteins on CD49f+/TROP2high basal PESCs. (A) FACS plot demonstrating the heterogeneous expression of CD13 within CD49fhigh expressing human basal PESCs, CD13‐APC=clone WN15; CD49f‐PE=clone GoH3, PI − negative gate, P < 0.001 as compared with Mouse IgG1 and Rat IgG2a isotype controls. (B) FACS plot demonstrating the heterogeneous expression of CD138 (Syndecan‐1, red colour as compared with isotype control=blue) and the correlation of higher CD138 expression with TROP2 expression in human basal PESCs. CD138‐PE=clone Wi15; TROP2‐APC=clone FAB650A (R&D), PI − negative gate, P < 0.001 as compared with Mouse IgG1 and Mouse IgG2a isotype controls. (C) FACS plots demonstrating the heterogeneous expression of SSEA‐1 on murine basal PESCs (below) and the heterogeneous expression of SSEA‐1 in correlation to the Sca‐1high expression of murine basal PESCs. SSEA‐1‐APC=clone MC480; Sca‐1‐PECy7 = clone E13‐161.7, PI − negative gate, P < 0.001 as compared with Mouse IgM and Rat IgG2a isotype controls. (D) FACS plots demonstrating the heterogeneous expression of SSEA‐4 on human basal PESCs. Around 20% of all human basal PESCs express SSEA‐4. SSEA‐4‐APC=clone MC813‐70; PI − negative gate, P < 0.001 as compared with Mouse IgG3 isotype control.