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. 2016 Nov 5;129(21):2623–2629. doi: 10.4103/0366-6999.192775

Figure 6.

Figure 6

Effect of DcR3 knockdown on the expressions of MMP9, VEGF-C, and VEGF-D of HepG2 was studied using Western blotting and RT-PCR. (a) Western blotting examined MMP9, VEGF-C, and VEGF-D protein levels in CON, NC, and DcR3-siRNA groups. House-keeping target GAPDH was included as the loading control. (b-d) RT-PCR determined significant downregulation of MMP9, VEGF-C, and VEGF-D mRNAs in HepG2 transfected with DcR3 shRNA group when compared to CON and NC groups. The pairwise comparisons between DcR3-siRNA and CON groups and between DcR3-siRNA and NC groups were statistically significant (P < 0.05). DcR3: Decoy receptor 3; RT-PCR: Real-time polymerase chain reaction; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; shRNA: Short hairpin RNA; mRNA: Messenger RNA; MMP6: Matrix metalloproteinase-9; VEGF: Vascular epithelial growth factor; CON: The blank control group; NC: Negative control group.