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Mesodermal three- lineage differentiation of hUCM-MSCs. Cells at passage 3 were cultured for 21 days in selective media for osteogenic, adipogenic and chondrogenic differentiation. (A) Osteogenic differentiation was examined by Alizarin red S staining. (B) Adipogenic differentiation was shown by oil red O staining. (C) Chondrogenic differentiation was analyzed by toluidine blue O staining
