Fig. 6.

The effect of 0, 30, 50 and 70 µM genistein on MMP2 caspase-3 enzyme activities in HT29 cells. Top: cells were incubated for 48 h and conditioned media were used for the measurement of MMP2 activity by gelatin zymography. The enzyme activity (MMP2) was reduced by increasing genistein concentrations. Bottom: caspase-3 activity was significantly increased with increasing genistein concentrations and the greatest enhancement in caspase-3 activity (89%) was observed in the presence of 70 µM genistein. One-Way-ANOVA analysis was performed to compare differences between groups and the symbol (*) represents significant differences (P< 0.05) between groups compared to untreated cells