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. Author manuscript; available in PMC: 2016 Nov 28.
Published in final edited form as: Sci Transl Med. 2016 Jun 8;8(342):342ra80. doi: 10.1126/scitranslmed.aaf1435

Fig. 5. siCAM5 reduces leukocyte recruitment into plaque.

Fig. 5

ApoE−/− mice were injected with either siCAM5 or siCtrl for 2 weeks. (A) Gating and quantification of GFPhigh myeloid cells in plaques and quantification of GFPhigh myeloid cells in blood after adoptive transfer of 2 × 106 GFPhigh Ly6Chigh monocytes and 2 × 106 GFPhigh neutrophils. Data are means ± SEM (n = 6 to 7 ApoE−/− mice per group from two independent experiments. (B and C) Gating and quantification of neutrophils, Ly6Chigh monocytes, and macrophages in atherosclerotic aortae (B) and in the blood (C). Data are means ± SEM (n = 6 to 10 ApoE−/− mice per group from three independent experiments). (D) Immunohistochemical staining of aortic roots for myeloid cells (CD11b) and neutrophils (Ly6G). Bar graphs show percentage of positive area per region of interest (ROI) or number of cells per high-power field (hpf). Scale bar, 250 μm. Data are means ± SEM (n = 5 to 6 ApoE−/− mice per group). P values in (A), (B), and (D) are determined by Mann-Whitney U test. (E) Quantification of neutrophils and Ly6Chigh monocytes in athero-sclerotic aortae after treatment with siCtrl, siVcam1, or siCAM5. Data are means ± SEM (n = 7 to 8 ApoE−/− mice per group from two independent experiments). *P < 0.05, ***P < 0.001, ****P < 0.0001, one-way ANOVA.