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. 2016 Jul 19;1(2):136–148. doi: 10.1002/btm2.10018

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© 2016 The Authors. Bioengineering & Translational Medicine is published by Wiley Periodicals, Inc. on behalf of The American Institute of Chemical Engineers

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Time course of immune cell infiltration into spinal cord bridges. Infiltrating cells form the central nervous system and peripheral immune system were identified in bridges implanted into a hemisection spinal cord injury (n = 6–21 mice) (A) CD45⁺ immune cell, GFAP⁺ astrocyte, and CNPase⁺ oligodendrocyte infiltration into bridges were quantified as a percentage of total cells using flow cytometry at days 7, 14, and 28. (B) Immune sub‐populations, including CD11c⁺ dendritic cells, F4/80⁺ macrophages, Gr‐1⁺ neutrophils, and CD4⁺ helper T cells, in the bridge were quantified as a percentage of total immune cells using flow cytometry at days 7, 14, and 28. Statistical analysis completed using a one‐way ANOVA with Tukey's post hoc test. Significantly different groups denoted by letter with “a” and “b” denoting statistical significance between the two groups (p <.05)