Figure 5.

IL‐10 alters macrophage morphology. Immunofluorescence (F4/80⁺/arginase⁺/Hoechst⁺: red/green/blue, respectively), was used to characterize the morphology of infiltrating macrophages within the bridge as either (A) fibrous, (B) round, or (C) FBGC (scale bar 50 µm). Arrows note arginase^‐ macrophages (white) and arginase⁺ macrophages (yellow). (D) Manual quantification of density (cells/mm²) of fibrous macrophages. (E) Manual quantification of density (cells/mm²) of round macrophages. (F) Manual quantification of density (fused nuclei/mm²) of FBGC macrophages. For all quantification, mean +/−SEM was plotted and n ≥3 where each replicate is an average of 1–4 tissues from an individual animal. Statistical analysis was completed on log‐normalized data using a two‐way ANOVA with Šidák correction for multiple comparisons with statistical significance indicated by a single asterisk (p < .05)