A. Hierarchy of chromosome structures: Chromosome territories, Topologically Associating Domains and insulated neighborhoods. Anchor refers to the CTCF-bound site interacting with another CTCF-bound sites, both co—bound by a cohesin ring.
B. Features of insulated neighborhoods in human embryonic stem cells (ESCs). The values displayed for the size range and number of genes represent the middle 95% of the data range.
C. Evidence for insulation of insulated neighborhoods: 97% Enhancer-gene interactions occur within insulated neighborhoods in human ESCs.
D. Evidence for insulation of insulated neighborhoods: Deletion of insulated neighborhood anchors leads to gene misregulation.
E. Evidence for insulation of insulated neighborhoods: Mutations of insulated neighborhood anchors in tumor cells leads to oncogene activation.