A. Targeting a dCas9-DNA-methyltransferase 3a/3l (Dnmt3a/3l) fusion to an insulated neighborhood anchor leads to DNA methylation, abrogation of CTCF binding and loss of neighborhood integrity. Black lollipops indicate DNA methylation.
B. Targeting a dCas9-TET (Ten-eleven translocation) fusion to an aberrantly methylated insulated neighborhood anchor leads to DNA de-methylation, and restoration of neighborhood integrity.