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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Cell. 2016 Nov 17;167(5):1188–1200. doi: 10.1016/j.cell.2016.10.024

Figure 8. Neighborhood perturbation and repair through site-specific DNA methylation.

Figure 8

A. Targeting a dCas9-DNA-methyltransferase 3a/3l (Dnmt3a/3l) fusion to an insulated neighborhood anchor leads to DNA methylation, abrogation of CTCF binding and loss of neighborhood integrity. Black lollipops indicate DNA methylation.

B. Targeting a dCas9-TET (Ten-eleven translocation) fusion to an aberrantly methylated insulated neighborhood anchor leads to DNA de-methylation, and restoration of neighborhood integrity.