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. Author manuscript; available in PMC: 2016 Nov 28.
Published in final edited form as: Methods Mol Biol. 2014;1102:461–480. doi: 10.1007/978-1-62703-727-3_24
Reagent/material Volume (μL) Final concentration
10× Titanium Taq buffer 2 1×
50× dNTP mix (10 mM) 0.4 0.2 mM
50× Taq polymerase 0.4 1×
MgCl2 (25 mM) 0.08 3.6 mMa
Genomic DNA (10 ng/μL) 2 1 ng/μL
Primer mix (2 μM) 2 0.2 μM
PCR-grade water 13.9 –
Total 20
a
Note: In addition to MgCl2 in 10× buffer for final concentration of 3.6 mM