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. Author manuscript; available in PMC: 2016 Nov 28.
Published in final edited form as: Methods Mol Biol. 2014;1102:461–480. doi: 10.1007/978-1-62703-727-3_24
Reagent/material Volume (μL) Final concentration
BigDye 3.1 (2.5×) 1 0.25×
BigDye buffer (5×) 1.5 0.75×
M13 F or R (10 μM) 0.32 320 nM
Cleaned-up PCR (1:50) 2 1:250
PCR-grade water 5.18 –
Total 10