ERBB4 Mutation Analysis: Emerging Molecular Target for Melanoma Treatment

. Author manuscript; available in PMC: 2016 Nov 28.

Published in final edited form as: Methods Mol Biol. 2014;1102:461–480. doi: 10.1007/978-1-62703-727-3_24

Step	Temperature	Time and cycles
1	96 °C	1 min
2	96 °C	10 s
3	50 °C	30 s
4	60 °C	4 min
5	–	Repeat steps 2–4 for a total of 25 cycles
6	4 °C	Hold