Table 3.
Teleost intestinal and systemic B cells and antibody responses after infection or mucosal vaccination. IHC: Immunohistochemistry; i.p.: intraperitoneal.
| Pathogen | Host (route administration) | B Cell regulation | Tissue analyzed | Procedure | Reference |
|---|---|---|---|---|---|
| Parasite | |||||
| Enteromyxum scophthalmi | Turbot | Upregulation of Ig-related genes expression | Pyloric caeca | RNA-seq | Robledo et al., 2014 |
| Increment of IgM+ B cells | Anterior intestine | IHC | Bermúdez et al., 2006 | ||
| Enteromyxum leei | Gilthead sea bream | Upregulation of IgM expression | Posterior intestine | RT-qPCR | Estensoro et al., 2012 |
| Increment of IgM+ B cells | Posterior intestine | IHC | Estensoro et al., 2012 | ||
| Upregulation of IgM and Ig-related genes expression | Posterior intestine | Microarray | Calduch-Giner et al., 2012 | ||
| Increment of IgM+ B cells | Posterior and anterior intestine | IHC | Estensoro et al., 2014 | ||
| Ceratonova shasta | Rainbow trout | Increment of IgT+ B cells | Midgut | IHC | Zhang et al., 2010 |
| Specific IgT in gut mucus | Gut mucus | ELISA | Zhang et al., 2010 | ||
| Chinook salmon | Increment of Ig+ cells | Midgut and anterior gut | IHC | Bjork et al., 2014 | |
| Bacteria | |||||
| Yersinia ruckeri | Rainbow trout (immersion) | Upregulation of IgM and IgT expression | Midgut | RT-qPCR | Evenhuis and Cleveland, 2012 |
| Aeromonas hydrophila | Carp (oral) | Specific IgM | Gut mucus | ELISA | Tu et al., 2010 |
| Seum | ELISA | ||||
| Aeromonas salmonicida | Rainbow trout (oral intubation) | Antibody-secreting cells | Intestine | ELISPOT | Davidson et al., 1993 |
| Flavobacterium psychrophilum | Rainbow trout (i.p.) | Upregulation of IgM expression | Midgut | RT-qPCR | Evenhuis and Cleveland, 2012 |
| Piscirickettsia salmonis | Atlantic salmon (oral boost) | Specific IgM | Serum | ELISA | Tobar et al., 2015 |
| Vibrio anguillarum | Turbot (oral) | Increment mIgM and sIgM expression | Hindgut | RT-qPCR | Gao et al., 2014 |
| African catfish (oral) | Specific IgM | Bile | ELISA | Vervarcke et al., 2005 | |
| Virus | |||||
| IPNV | Rainbow trout (antigen oral) | Increment IgT+ and IgM+ cells | Pyloric caeca | IHC | Ballesteros et al., 2013 |
| Rainbow trout (antigen oral) | Upregulation IgM and IgT expression | Pyloric caeca | RT-qPCR | Ballesteros et al., 2013 | |
| Rainbow trout (infection oral) | Increment IgM+ cells | Pyloric caeca | IHC | Ballesteros et al., 2014 | |
| CHNV | Carp (oral) | Specific Ig response | Serum | ELISA | Sato and Okamoto, 2010 |
| ISAV | Atlantic salmon (oral boost) | Specific IgM | Serum | ELISA | Tobar et al., 2015 |