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. Author manuscript; available in PMC: 2017 Nov 21.
Published in final edited form as: Dev Cell. 2016 Nov 3;39(4):424–437. doi: 10.1016/j.devcel.2016.10.006

Figure 2. Removal of sDPA-associated components from both ends of centrioles.

Figure 2

(A) C-Nap1 is necessary to target each member of the Ninein Group to the proximal ends of centrioles. WT or C-Nap1 knockout (KO) cells were stained with indicated antibodies. Arrows indicate the mature mother centrosome.

(B, C and D) The localization of each Ninein Group protein in RPE1 cell lines depleted of indicated proteins by CRISPR is shown.

(E) Rescue of ninein localization. CEP128 was exogenously expressed in RPE1 cells that lacked both endogenous CEP128 and C-Nap1. Also shown included the restoration of C-Nap1 expression in CEP128 and C-Nap1 double KO cells by a second round of CRISPR-mediated gene editing (see Experimental Procedures).

(F) A full schematic of the assembly hierarchy of sDAP components.