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. 2016 Nov 28;14(11):e2000998. doi: 10.1371/journal.pbio.2000998

Fig 5. KSHV miRNAs increase expression of miRNA biogenesis components Dicer and TRBP.

Fig 5

(A) Dicer protein expression was determined by western blot from HUVECs transfected with individual KSHV miRNA mimics. The results are averaged from 4–5 replicates and a representative western blot is shown. (B) TARBP2 RNA expression was determined using qPCR from HUVECs transfected with individual KSHV miRNA mimics. Results are shown relative to β-actin and normalized to the negative control. For all graphs, results are shown as mean ± SD, N = 3. Significance was assessed using a Student’s t test, *p ≤ 0.05, **p ≤ 0.01. (C) Dicer and TRBP expression was measured using qPCR as in (B) from iSLK cells containing a WT KSHV genome and a mutant containing a KSHV miRNA cluster deletion. N = 5. For all graphs, results are shown as mean ± SD. Significance was assessed using a Student’s t test, *p ≤ 0.05, **p ≤ 0.01. Numerical data can be found in S1 Data.