Table 1. Summary of accuracy, precision, recovery and matrix effect of the eleven analytes in rat prostate (n = 6).

Analyte	Concentration (ng/ml)	Intra-day RSD (%)	Inter-day RSD (%)	Accuracy (RE %)	Recovery (%, mean ± SD)	Matrix effect (%, mean ± SD)
15-HETE	2.5	6.1	4.1	1.5	94.3 ± 8.4	86.3 ± 2.7
	25	6.5	1.9	−7.2	93.4 ± 1.9	109.9 ± 4.0
	400	1.5	8.1	3.9	97.6 ± 3.9	99.6 ± 4.7
12-HETE	0.5	7.5	4.8	1.4	96.7 ± 2.8	91.6 ± 4.2
	5	4.9	0.8	−5.4	74.8 ± 3.1	89.3 ± 6.6
	80	9.2	7.2	−3.5	77.7 ± 3.4	86.2 ± 3.1
TXA2	1.25	9.5	5.3	−2.9	94.4 ± 4.5	105.7 ± 6.9
	12.5	6.2	8.6	4.7	95.5 ± 9.3	88.1 ± 7.4
	200	4.6	3.5	−0.8	84.3 ± 6.2	86.2 ± 5.3
5-HETE	1	6.0	8.3	2.5	79.4 ± 0.9	100.4 ± 0.9
	10	2.9	9.8	2.5	89.6 ± 8.4	106.8 ± 5.8
	160	8.3	8.9	2.0	73.7 ± 8.6	101.5 ± 0.3
AA	1 00	6.0	8.5	−4.4	72.5 ± 7.0	93.6 ± 6.7
	10 00	9.8	6.8	−0.7	92.2 ± 5.4	99.1 ± 7.3
	1 6 000	3.6	4.5	0.5	81.2 ± 5.3	107.9 ± 2.8
PGI2	2	1.9	10.1	−3.7	93.9 ± 2.0	104.9 ± 4.9
	20	0.5	4.5	0.3	84.7 ± 3.1	86.4 ± 7.1
	320	1.9	2.1	0.4	78.3 ± 3.7	95.7 ± 2.5
PGF2α	2.5	5.8	6.7	−4.0	98.6 ± 6.9	107.8 ± 9.4
	25	6.5	6.1	−3.5	87.8 ± 7.9	86.9 ± 8.4
	400	9.1	2.1	1.0	91.3 ± 6.9	104.7 ± 3.3
8-HETE	1.25	5.0	1.3	6.1	88.0 ± 6.4	90.6 ± 8.8
	12.5	4.0	4.3	−0.1	90.7 ± 5.8	105.2 ± 9.6
	200	9.6	9.2	−0.8	92.9 ± 6.6	94.9 ± 7.2
PGD2	2.5	2.7	3.5	0.1	98.5 ± 4.5	107.9 ± 7.4
	25	9.0	8.3	4.4	82.4 ± 8.4	85.9 ± 6.2
	200	8.5	8.6	0.6	97.5 ± 4.0	102.1 ± 9.6
PGE2	2	2.0	2.5	−3.9	75.7 ± 2.2	85.7 ± 9.0
	20	9.3	9.7	−4.6	94.8 ± 2.3	92.1 ± 2.3
	200	5.3	9.4	3.2	72.1 ± 6.4	105.7 ± 1.8
LTB4	2.5	9.0	3.8	−8.5	84.4 ± 9.1	100.9 ± 8.8
	25	7.1	2.9	4.0	92.4 ± 7.2	100.5 ± 6.8
	400	5.2	2.1	−0.8	94.8 ± 7.6	93.0 ± 5.1