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. 2016 Nov 28;11(11):e0166935. doi: 10.1371/journal.pone.0166935

Fig 3. Characterization of Siglec-9-EC with the fluorescence-based thermal shift assay.

Fig 3

(A) The effect of different buffer pH values on the stability of Siglec-9-EC. The tested buffers were: Citric_pH4 = 100 mM citrate buffer pH 4.0, NaAcetate_pH5 = 100 mM Sodium acetate buffer pH 5.0, MES_pH6 = 100 mM MES buffer pH 6.0, HEPES_pH7 = 100 mM HEPES buffer pH 7.0, Tris-HCl_pH8 = 100 mM Tris-HCl buffer pH 8.0, ImidMaleic_pH 8.5 = 100 mM N-imidazolyl maleamic acid pH 8.5, CHES_pH 9.5 = 100 mM CHES buffer pH 9.5. All of them contained 125 mM NaCl. (B) Thermal shift assay of Siglec-9-EC in 20 mM HEPES buffer, 150mM NaCl, pH 7.4 in the presence of different additives. (C) Thermal shift assay of Siglec-9-EC and the mutant Siglec-9-EC proteins to check the effect of the mutations on the stability of the protein.