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. 2016 Nov 28;11(11):e0166935. doi: 10.1371/journal.pone.0166935

Fig 6. The role of sugars, sialic acid binding domain (ΔV) and the mutations R284S, R290S on the binding of Siglec-9-EC to hAOC3.

Fig 6

(A) Sialic acid addition decreases the binding of Siglec-9-EC (WT) to hAOC3 by SPR. (B) The effect of the disialyl lactotetraosylceramide (DSLc4) sugar on hAOC3–Siglec-9-EC interaction. Each experiment was performed as duplicate. (C) Relative binding of Siglec-9-EC (WT), -R284S, -R290S, -ΔV, -R120S, R120S/R284 and–R120S/R290S mutants at concentration of 0.5 μM to immobilized hAOC3 (means of two (R120S based mutants)-three experiments are shown ± SEM). *: p<0.05, **:p<0.01 and ***:p<0.001.