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. 2016 Nov 28;11(11):e0166932. doi: 10.1371/journal.pone.0166932

Fig 7. UV cone ablation does not cause alterations in the abundance of non-target cone subtypes.

Fig 7

(A-F) Transgenic zebrafish retina labelled with antibodies against double cones. Top Row: Treated with vehicle control DMSO; Bottom Row: treated with prodrug metronidazole (MTZ) to induce ablation of UV cones. Retinas are visualized as whole mounts, wherein double cones are labeled with antibody zpr1, and UV cones are filled with mCherry (pseudocoloured to cyan) fused to nitroreductase. Note that in (E) UV cones are greatly reduced in abundance due to application of prodrug MTZ. (C, F) Merged image showing the larval mosaic. Scale bar is 50 μm. (I) The numbers of UV and double cone photoreceptors obtained from DMSO and MTZ treated zebrafish larvae are indistinguishable, i.e. ablating blue cones does not detectably disrupt adjacent cones. n = 6 or 5 fish for DMSO and MTZ treatments, respectively.