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. 2016 Nov 28;11(11):e0166938. doi: 10.1371/journal.pone.0166938

Fig 8. Detection of TSWV levels in infected Money Maker, NahG and BTH-treated NahG plants.

Fig 8

Quantitative ELISA to detect TSWV at 0.5, 1 and 2 wpi. A polyclonal antibody against TSWV (BR-01, Loewe) and an alkaline phosphatase conjugated antibody were used as primary and secondary antibodies, respectively. Immunodetection was carried out by using p-nitrophenyl phosphate as a substrate and the absorbance was measured at 405 nm. Data correspond to the mean ± SD of three biological replicates of one representative experiment.