Fig 5. Assessment of haptocorrin and transcobalamin expression in HT1080 cells and growth medium.

A, HT1080 cells were cultured in DMEM containing either 10% FCS, 10% heat-treated (95°C for 10 min) FCS, or 10% HS, for 2 h, 12 h, 24 h and 48 h and the cells, and B, cell culture medium supernatants, were collected and analysed for haptocorrin (HC), transcobalamin (TC), and β-actin by western blotting. Positions of molecular weight markers (kDa) are given on the right side of the blots. Ponc., Ponceau red stained membrane.