Fig 1. Gene constructs for antibody and protease inhibitor expression in N. benthamiana leaves.

The diagram identifies coding sequences of individual H10 antibody heavy and light chains for insertion in a pBI expression vector, and those of the accessory protease inhibitors α₁-ACT, SlCYS8 and SlCYS8 inactive mutant Q47P for insertion in a pEAQ vector. All coding sequences were placed under the control of a Cauliflower mosaic virus 35S promoter (35S) and an Ω or CPMV-HT translational enhancer sequence. L, signal peptide for antibody chain cellular secretion, from the heavy chain of an embryonic mouse immunoglobulin; PDI, signal peptide of an alfalfa protein disulphide isomerase; NOS, nopaline synthase terminator sequence.